Answer to Question #110794 in Molecular Biology for FRIDAY

Question

Question 5: As previously stated, the concentration of NaCl, KCl and Phosphate in working strength 1 x PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4 How do they compare to the concentrations you calculated for 10x PBS?

Answer 1

The concentrations of NaCl, KCl, and phosphate in 1xPBS are ten times lower than in 10xPBS. As a result, in 10xPBS, the concentrations are 1.37M NaCl, 0.12M Phosphate, and 0.027M KCl.