If the following sequence of bases represents the start of a gene on a double stranded DNA, what is the sequence of the transcribed RNA, what is its polarity , and what is the polarity of the DNA?
G C T A C G G A T T G C T G
C G A T G C C T A A C G A C
Q#1: Why two metal ions (Mg2+) participate in DNA polymerase reaction and why not in DNA polymerase?
Q#2: What is the distance between nucleotide -10 and -35 sequence and for which activity it is important?