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i used to wash my mouth with 70% ethanol and hold it in my mouth for 5 minutes or so. i do it no more but iam concerned about it causing cancer through its metabolite Acetaldehyde.. there ist something strange... i never felt neither the symptoms of excess ethanol nor the symptoms of excess Acetaldehyde, although the mount i used was nearly 30 ml.. but i sprayed it over the upper and lower lip.. could that be because the Ethanol and Acetaldehyde in it evaporate before they reach my mouth or very soon after they land inside my mouth????


  1. if you want to overexpress a protein in ecoli. The protein is from human ocular cells. What vector would you use to express the protein?. You know the sequence of the gene for the ocular protein, where would you get the DNA you need to clone into the vector? And what regulatory sequences need to be present in your vector for expression to occur?
  2. how will u make a conditional mutant in mice using cre/lox system?

thank you


I want to know the general concentration of catalase in Brócoli, potatoes, onions, carrots and cucumbers


Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.


Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.


If the transcription factor is reduce. Can the hypothesis be solve this issue by upregulating TF


If by mutation or aging if the transcription factor diminish.....then is there a way to upregulate the transcription factor


Only one strand of the double helix is used as template for transcription. How would the transcription machinery recognize the template strand for transcription of a particular gene? Is it necessary that this strand will be used as template for transcription of all genes of that particular organism?

Suppose we have a genomic DNA of 1000 kb. It gives two products: 1000 kb product and 200 kb product. What can you infer about the nucleic acid nature of each product? Which process will give the 1000 kb product and which will generate the 200 kb product?   

Suppose we have a consensus sequence “5′-GTAATC-3′” at a particular site in the genome of a specie. Does this mean that all members of that specie will have the sequence “5′-GTAATC-3′” at that particular site? If yes, explain. If no, why?

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