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What could potentially happen to the organism if the IHF protein loss its function?
Thanks in advance!
glucose, 5 grams (g); NH4Cl, 1g; KH2PO4, 1g; MgSO4, 0.3g; yeast extract, 5g;
distilled water, 1 litre
(1) Gel filtration chromatography
(2) Paper chromatography
(3) Affinity chromatography
(4) Thin layer chromatography
My attempt at a solution:
Well, the analyte contains salt (possible inorganic so the particles, ions will be small enough as compared to a protein) and protein (a large one) so SEC or gel filtration looks perfect.
But there's affinity chromatography too. Two things come to my mind: 1.having an antibody or enzyme in the stationary phase will definitely work at holding back the proteins 2. but there may be some non specific polar interactions between the protein and the ions, so... (1) 's the right one?