Answer to Question #110722 in Molecular Biology for FRIDAY

Question #110722
Question 2: Based on the equation below, which ion plays the role of hydrogen-ion donor (acid) and which ion plays the role of hydrogen-ion acceptor (base) in PBS?
H2PO4-(aq) ⇆ H+(aq) + HPO42-(

The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS.
Combine the following:
• 80g NaCl
• 2g KCl
• 14.4g Na2HPO4 (dibasic anhydrous)
• 2.4g KH2PO4 (monobasic anhydrous)
• 800mL distilled H2O

1. Adjust pH to 7.4 with HCl
2. Add H2O to 1L
3. Autoclave for 20 minutes on liquid cycle. Store at room temperature.
Expert's answer

The reaction given:

H2PO4-(aq) ⇆ H+(aq) + HPO42-( aq)

Here, H2PO4- ion serves as a proton donor, while HPO42- ion is a proton acceptor. In a PBS solution, Na2HPO4 dissociates into Na+ and HPO42-. As a result, this molecule serves as a proton acceptor. In contrast, KH2PO4 dissociates into K+ and H2PO4-. As H2PO4- is a proton donor, KH2PO4 serves as a proton donor in a PBS solution.

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