58 669
Assignments Done
Successfully Done
In March 2018
Your physics homework can be a real challenge, and the due date can be really close — feel free to use our assistance and get the desired result.
Be sure that math assignments completed by our experts will be error-free and done according to your instructions specified in the submitted order form.
Our experts will gladly share their knowledge and help you with programming homework. Keep up with the world’s newest programming trends.

Answer on Biochemistry Question for Maria

Question #11129
Explain the effect of increasing and decreasing the pH on the electrophoresis process
Expert's answer
Electrophoresis is a method for separating substancesaccording to their charge. Proteins, nucleic acids and other biomolecules have different charged groups (such as -NH3[+], -PO4[2-], -SO3[-]) that led to a movement of biomolecules in the electric field. Changes in solution pH result in changes of charge of the biomolecules, because of this results of electrophoresis will be different in different pH. I.e. if we increase solution pH, -NH3+ groups loose their charge and turn to -NH2 groups, thus a molecule will decrease its charge and will move slower during electrophoresis. On the contrary -SO3 groups are uncharged in the low pH, but obtain negative charge in the high pH (thus molecule with large amount of -SO3 groups will move during electrophoresis only in solutions with high pH).

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!


No comments. Be first!

Leave a comment

Ask Your question