A mutant has shown the ability to break down starch into simple sugar and convert the simple sugar into a molecule named brooklynamide (bright red). After investigation, the scientists found out that the mutant has a gene insertion, Gene A, which transcribe and translate into Protein A; and Gene B for Protein B.
The scientists successfully extracted protein A. They hypothesized that Protein A is responsible for the breaking down of starch while Protein B is responsible for synthesizing brooklynamide. As part of the research team, you are tasked to design a simple experiment to prove the hypothesis.
After proving the above, scientists found out that protein B is, in fact, an enzyme and named it brooklynamide synthase. However, none of its properties was known. Design experiment to determine:
a) Enzyme’s optimal pH
b) Enzyme’s optimal temperature
c) Minimum enzyme concentration to reach maximum rate of reaction