Answer to Question #133405 in Inorganic Chemistry for Darius

Question #133405
Six litres of 0.4M 3-(N-morpholino) propane sulfonic acid (MOPS) buffer of pH 7.8 are required. Available in the laboratory are 10M HCl and 10M NaOH and the zwitterionic form of MOPS (M.Wt = 210.3). The pKa for the MOPS zwitter ion is 7.2. Describe, showing the relevant calculations, the procedure for preparing the buffer . If 10ml of 10M HCl was added to 1 litre of the above buffer (question 9) what would be the resulting pH?
What would be the resulting pH if 10ml of 10M HCl were added to 1 litre of pure water?
Expert's answer

The following formula is used to calculate the pH of the given buffer

Rearranging the formula

The concentration of the buffer solution is

The concentration of MOPS and Na-salt required can be now evaluated

Calculate the number of moles of HA and BA

The total number of moles of the solution is

Mass of MOPS=

Volume of NaOH=1.92/10=0.192 L

Mix 504.7 g of MOPS with 0.192 L of 10M NaOH solution

In a flask, dissolve this mixture with distilled water up to 6L after the reaction is complete

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