Answer to Question #1650 in Molecular Biology for Emily

Question

Design a set of Prime that can be used to PCR amplify the target DNA sequence indicated by the dots?

5’ GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT 3’
3’ CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA 5’

If a single DNA molecule is amplified by PCR, how many DNA molecules will there be after 6 cycles?

Answer 1

The forward and reverse primers would be:
5’…GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT …3’
CCGATTCTAGACTTAAAA
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GCAATAATGTAGCGCCTT
3’…CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA… 5’
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After one cycle of PCR we get 2 molecules of DNA. So after 6 cycles we get 26 = 64 DNA molecules.