Question #1650

Design a set of Prime that can be used to PCR amplify the target DNA sequence indicated by the dots?
5’ GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT 3’
3’ CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA 5’
If a single DNA molecule is amplified by PCR, how many DNA molecules will there be after 6 cycles?

Expert's answer

The forward and reverse primers would be:

5’…GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT …3’

CCGATTCTAGACTTAAAA

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GCAATAATGTAGCGCCTT

3’…CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA… 5’

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After one cycle of PCR we get 2 molecules of DNA. So after 6 cycles we get 26 = 64 DNA molecules.

5’…GGCTAAGATCTGAATTTTCCGAG......TTGGGCAATAATGTAGCGCCTT …3’

CCGATTCTAGACTTAAAA

---------------------------------------------------------------------

GCAATAATGTAGCGCCTT

3’…CCGATTCTAGACTTAAAAGGCTC.......AACCCGTTATTACATCGCGGAA… 5’

---------------------------------------------------------------------

After one cycle of PCR we get 2 molecules of DNA. So after 6 cycles we get 26 = 64 DNA molecules.

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