Answer to Question #1885 in Molecular Biology for zara
You generate a mutant form of Ran that can not bind NTF2, but is capable of performing all other Ran related activities . if you express a GFP - tagged version of this protein in cells, predict where the protein will accumulate over time and indicate what Nucleotide- bound state it will be in .
Ran remains concentrated in the nucleus when importin-mediated protein import is disrupted and that NTF2 does not form a stable complex with the transport receptor, importin-β. GFP remains concentrated in the nucleus.