You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to an
enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.
Polymerase Chain Reaction
After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR
using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer
briefly but completely.
a. DNA polymerase isolated from Thermus aquaticus
b. Deoxynucleotide triphosphates (dNTPs)
c.Forward and reverse primers
a) directly participates in the synthesis of DNA strands that are complementary to template molecules at a very high temperature that reaches 97°C;
b) are the main building blocks for the synthesis of DNA molecules;
c) attach to the start codons of antisense and sense chains of template molecules respectively, giving the direction in which DNA polymerase will synthesize DNA chains from dNTPs.