Answer to Question #237800 in Cell Biology for Shelly

Question #237800

 The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4.

 Combine the following:

·        80g NaCl

·        2g KCl

·        14.4g Na2HPO4 (dibasic anhydrous)

·        2.4g KH2PO4 (monobasic anhydrous)

·        800mL distilled H2O

1.     Adjust pH to 7.4 with HCl

2.     Add H2O to 1L

3.     Autoclave for 20 minutes on liquid cycle. Store at room temperature.

Which ions are being produced by this process,

Expert's answer

Mixing the given reagents to prepare a phosphate-buffered saline (PBS) solution involves the following dissociation reactions in water: 

  • NaCl(aq) ----------> Na(aq)+Cl(aq) 
  • KCl(aq) ---------->K+(aq) + Cl(aq) 
  • Na2HPO4(aq) ----------> 2Na(aq) + HPO2−4 (aq) 
  • KH2PO(aq) ----------> K(aq) + H2PO−4(aq) 
  • HCl (aq) +H2O (l) ----------> H3(aq) + Cl (aq) 

Based on the chemical equations above, we can see that the preparation process produces the following ions once the reagents are dissolved in water:  Na+ ,  K+ , Cl- , HPO2-4 ,  H2PO-4 , H3O+ .

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