Answer to Question #5087 in Biochemistry for saif
So, the total number of hydrogen atoms in the palmitate is 31. If we use protonated form of palmitic acid (with hydrogen in carboxylic group), the number is 32. But it must be stressed, that all forms of hydrogen (for example, NADPH+H+, H2O) that are used in the enzymatic synthesis of palmitate also must contain 3H-isotope. If not, the total number of incorporated 3H will be significantly less.
Tritium is a “heavy” isotope of hydrogen. It has 2 neutrons in the nucleus, so the mass of tritium atom (3H) is 3 Da instead of 1 Da of 1H.
Answer: the total number of hydrogen atoms in the palmitate is 31. If we use protonated form of palmitic acid (with hydrogen in carboxylic group), the total number is 32.
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