Answer to Question #265466 in Biochemistry for casanova

Question

You are a team of biochemists, botanists, and microbiologists who were tasked to isolate and identify antimicrobial peptides (AMPs) from endemic plants in the Philippines. In one of your studies, you were able to isolate 4 AMPs from a certain shrub in the mountainous area of Cebu, where 3 of them consists of amino acids only while the last one has a carbohydrate moiety. The AMPs were tested using amino acid analyzer and found out the following sequences for the 4 peptides

Amino Acid Sequence

1. Val-Gly-Leu-Phe-Asp-Phe-Val-Phe-Tyr-Gly-Ser-Phe (VGLFDFVFYGSF)
2. Asn-Pro-Cys-His-Glu-Ala-Cys-Cys-Ser-Cys-Leu-Cys (NPCHEACCSCLC)
3. Gly-Gly-Leu-Trp-His-Ala-Val-Trp-Thr-Trp-Phe-Ala-Trp (GGLWHAVWTWFAW)
4. (with carbohydrate moiety) Ser-Leu-Glu-Lys-Gly-Leu-Asp-Gly-Ala-Lys-Lys-Ala (SLEKGLDGAKKA)

Now create a procedure using different color reactions that would easily distinguish the four peptides from one another

II. Hypothesis-Testing Method Design

A. Problem

B. Theory

C. Hypothesis

D. Procedure

E. Results and Discussion

F. Conclusion

Answer 1

Antimicrobial peptides aggregate with each other, penetrate the bilayer of the cell membrane in the form of multimers, and form channels that result in the cytoplasmic outflow. In severe cases, AMPs can induce cell membrane collapse and lead to cell death