Answer to Question #265458 in Biochemistry for casanova

Question #265458

A sample containing mixture of methionine, proline, cysteine, histidine, and glutamic acid was subjected to electrophoresis at pH 4. Show the migration of each amino acid in the electrophoresis matrix by drawing spots corresponding to the location of each amino acid. Label each the spots with the 3-letter abbreviation of the amino acid. Show the calculation of pI of each amino acid below. The ionization of each amino acid is no longer necessary to be illustrated.


1
Expert's answer
2021-11-16T08:05:01-0500

For the acidic amino acids, calculate the percentage that are charged by taking one minus the proportion with H associated. Multiply the proportion charged by the number of each amino acid present in the protein. Subtract the negative charge total from the positive charge total to get the net charge.


Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!

Comments

No comments. Be the first!

Leave a comment

Ask Your question

LATEST TUTORIALS
New on Blog
APPROVED BY CLIENTS