Question #71282
The molar absorptivity of NADH is 6.22x10³ L. mol¯¹. cm¯¹ at 340 nm. In an alanine transaminase assay mixture of 1.0 ml, containing 20 µl serum, a change in absorbance at 340 nm of 0.600 is found after 5 minutes incubation in a 1 cm cuvette. Calculate the alanine transaminase activity of the serum.
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