Answer to Question #66020 in Chemistry for Jade

Question

Hi
I would like to know how to draw a Lowry assay graph

Answer 1

Answer:
Lowry’s assay for total protein is one of the most commonly performed colorimetric assays.
This procedure is sensitive because it employs two colour forming reactions. It uses the Biuret reactions in which Cu2+ in presence of a base reacts with a peptide bond of protein under alkaline conditions resulting in reduction of cupric ions (Cu2+) to cuprous ions (Cu+), and Lowry’s reaction in which the Folin Ciocaltaeu reagent which contains phosphomolybdic complex which is a mixture of sodium tungstate, sodium molybdate and phosphate, along with copper sulphate solution and the protein, a blue purple colour is produced which can be assessed by measuring the absorbance at 650-700 nm .
It is based on photocolorymetric measurements of total protein in the solutions with a known concentration with an indication of the corresponding electrical signal.
When total protein concentration is measured in the solution with unknown concentration, the signal is projected to the earlier built assay graph and the concentration is found out.