Tris(hydroxymethyl)aminomethane (or THAM) is a weak base frequently used to prepare buffers in biochemistry. Its pKb is 5.92. The corresponding pKa is 8.08 near the pH of physiological buffers, thus it exhibits good buffering capacity at physiological pH. What is the mass of THAM must be taken with 100mL of 0.50M HCl to prepare 1 L of a pH 7.40 buffer ?
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