1. Sanger’s method for N-terminal residue analysis involves treating a peptide with 1- fluoro-2,4-dinitrobenzene, which is very reactive toward nucleophilic aromatic substitution. 1-fluoro-3,5-dinitrobenzene does not react.
2. Describe ONE experiment that can be used to confirm the proposed E1cB and disprove both the E1 and E2 mechanisms.
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