Answer to Question #286209 in Organic Chemistry for Alia

Question

Suggest a suitable ion exchange chromatography to purify proteins A, B, C, and D from other proteins in the mixture. Explain your suggestion and discuss briefly the principle of the separation.

Answer 1

Among all chromatographic techniques, affinity chromatography plays a major role. In fact, affinity chromatography is the most specific and effective protein purification technique, providing a rational basis for the purification of target proteins.

Separation of powers is a doctrine of constitutional law under which the three branches of government (executive, legislative, and judicial) are kept separate. Each branch has separate powers, and generally each branch is not allowed to exercise the powers of the other branches.