Answer to Question #199931 in Organic Chemistry for Sankalp bagde

Question #199931

An oligopeptide was analyzed. Given the data below, what is its sequence? • AA analysis revealed the composition Asp Asn Glu2 Gly Lys Met2 Phe Pro2

. Carboxypeptidase digestion gave the results: Gly

Sanger N-terminal analysis afforded the DNP derivative of glutamic acid

Treatment of the peptide with cyanogen bromide gave three fragments. Sanger N terminal analysis of these three fragments gave the DNP derivatives of Glu, Pro, and Asp.

• Cleavage of the oligopeptide with trypsin gave two fragments. Sanger analysis of

these two fragments both gave the DNP derivative of Glu • Cleavage of the oligopeptide with chymotrypsin gave two fragments. Sanger analysis

of these two fragments gave DNP derivatives of Glu and Lys

Expert's answer

The N terminal amino acid of the protein is labeled by Sanger's reagent. ... The labeled protein is then hydrolyzed and loaded on chromatography column and the elution profile is matched with the standard profile obtained from FNB derivative of all the amino acids

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