Answer to Question #23082 in Inorganic Chemistry for Ebenezar stanley
Beer's Law follows:
Aλ = ελbc
ελ = molar absorptivity or extinction coefficient of the chromophore at wavelength λ (the optical density of a 1-cm thick sample of a 1 M solution). ελ is a property of the material and the solvent.
b = sample pathlength in centimeters
c = concentration of the compound in the sample, in molarity (mol L-1)
In an absorbance experiment, light is attenuated not only by thechromophore, but also by reflections from the interface between air and the sample, the sample and the cuvette, and absorbance by the solvent. These factors can be quantified separately, but are often removed by defining I0 as the light passing through a sample "blank" or "baseline" or reference sample (for example, a cuvette filled with solvent but zero concentration of the chromophore is used as the blank).Many factors can affect the validity of Beer's Law. It is usual to check for the linearity of Beer's Law for a chromophore by measuring the absorbance of a series of standards. This "calibration" can also remove errors in the experiment, the equipment, and the batch of reagents (such as cuvettes of unknown pathlength).
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