Answer to Question #184265 in Inorganic Chemistry for Zainab

Question #184265

Describe how you will determine concentration of tryptophan in grains of soy bean harvested from field fertilised with NPK 15-15-15 using UV/VIS spectroscopy


1
Expert's answer
2021-04-23T07:31:29-0400

Using UV detection for amino acids in most cases requires using the absorption of the carboxyl group (-COOH) in the 200 to 210 nm range.

Tryptophan will absorbs more light at 280nm


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