Answer to Question #57243 in General Chemistry for Sabrina Ahmad
1. I need to prepare 50 mM of sodium acetate buffer at pH 5.4 and then adjust its molarity to 0.1, 0.2 and 0.5 M using NaCl alone. Suppose I prepare 1 L of the buffer, I should add 4.1 grams of sodium acetate to get a molarity of 50 mM. Now how do I calculate the amount of NaCl to be added to the buffer to get the desired molarities?
2. I am trying to reproduce a research paper and the amount of protein (bovine serum albumin) mentioned in the paper is in volume fractions 0.1, 0.2, 0.3 and 0.4 added to the sodium acetate buffer. I am having trouble calculating the amount of protein which needs to be added. I have 10 grams of the protein. Is there any formula to convert volume fraction into the weight in mg?