Answer to Question #210594 in General Chemistry for thi@

Question #210594


 The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 liter of 10x concentrate PBS.

 Combine the following:


1
Expert's answer
2021-06-25T06:42:16-0400

The reaction given:

H2PO4-(aq) ⇆ H+(aq) + HPO42-( aq)

Here, H2PO4- ion serves as a proton donor, while HPO42- ion is a proton acceptor. In a PBS solution, Na2HPO4 dissociates into Na+ and HPO42-. As a result, this molecule serves as a proton acceptor. In contrast, KH2PO4 dissociates into K+ and H2PO4-. As H2PO4- is a proton donor, KH2PO4 serves as a proton donor in a PBS solution.


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