Answer to Question #209731 in General Chemistry for Milse

Question

Solution A: Nanoparticles at 10mg/ml (1.3x10^12particles/ml) Solution B: 150 kDa Antibody at 12uM Solution C: Diluent solution Solution D: Sample And I told you I wanted to perform an experiment in which 5ul of the sample that we are testing is incubated in a solution containing 250pM Nanoparticles, 600nM antibody, and is at least 30% diluent solution, and had a final volume less than 12ul. How would you setup this incubation?

Answer 1

Optimum temperature: 100.5 degrees Fahrenheit. Temperaturerange: 99-102 degrees Fahrenheit. humidity, day. 12 + 5ul= 17ul

250pM

10 × 1.3 × 10^14

= 1.3 × 10^14 particles/ml