Answer to Question #186565 in General Chemistry for rashith

Question

2. Sample Calculations

From the data contained above in Table 2 above, and using the formula shown on Page 5, the following table below can be constructed. You must show in a sample calculation how you determined one set of values shown in Table A. 

2.1. Determine the amount (mg) of Carboxylesterase enzyme present in 50 μL (1 mark)

 

 

 

 

 

 

 

 

2.2. Determine p-npa substrate concentration (2 marks)

 

 

 

 

 

 

 

 

 

2.3. Velocity (v*) (2 marks)

 

 

 

 

 

 

 

2.4. 1/v* (1 mark)

 

 

 

 

 

 

 

 

2.5. 1/[p-npa] (1 mark)

 

               

 

 

 

 

 

 

 

 

Answer 1

I cannot find any parameters to solve for the mass of enzyme present

However, look for the number of moles by multiplying volume by concentration and then multiply the result with the molar mass of the enzyme