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Answer to Question #89551 in Other Biology for Rachel

Question #89551
An experiment is investigating the effect of an enzyme on a substrate in a buffered solution. Which of the following is an example of an appropriate negative control condition?
A) Substrate and buffer
B) Substrate, buffer and a different enzyme
Expert's answer

A) Substrate and buffer.

Negative control represents a reaction where the enzyme is absent, or it is not working. The reaction should be characterized by the absence of any changes in the substrate concentration. As a result, the substrate in the buffer solution without any enzyme must be used as a negative control.

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