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Answer to Question #81496 in Biology for Rahma
Question #81496
How can you make a buffer in the lab?
Expert's answer
Answer:
There are several ways to make buffer solution in the lab:
1. Using a conjugate acid-base pair with a pKa the same as the desired pH.
2. Using a conjugate acid-base pair with a pKa close to the desired pH and adding a strong acid or base to get the exact pH required
3. Using a weak acid and partially titrate with a strong base or using a weak base and partial titration with a strong acid.
There are several ways to make buffer solution in the lab:
1. Using a conjugate acid-base pair with a pKa the same as the desired pH.
2. Using a conjugate acid-base pair with a pKa close to the desired pH and adding a strong acid or base to get the exact pH required
3. Using a weak acid and partially titrate with a strong base or using a weak base and partial titration with a strong acid.
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