58 602
Assignments Done
Successfully Done
In March 2018
Your physics homework can be a real challenge, and the due date can be really close — feel free to use our assistance and get the desired result.
Be sure that math assignments completed by our experts will be error-free and done according to your instructions specified in the submitted order form.
Our experts will gladly share their knowledge and help you with programming homework. Keep up with the world’s newest programming trends.

Answer on Other Biology Question for James Cagri

Question #67332
What criteria do restriction enzymes have to meet when deciding which to choose in order to clone a specific gene into the multiple cloning site of a plasmid (e.g. pUC18)?
Expert's answer
The main criteria restriction enzymes must meet for the application in cloning a specific gene into the multiple cloning site of a plasmid are:

- Flank the insert, but does not cut within the insert;
- Is in the desired location in the recipient plasmid;
- Results in the insert being in the correct orientation in the recipient plasmid;
- Is in the frame with tags or fusion proteins in the recipient plasmid.

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!


No comments. Be first!

Leave a comment

Ask Your question