58 830
Assignments Done
Successfully Done
In March 2018
Your physics homework can be a real challenge, and the due date can be really close — feel free to use our assistance and get the desired result.
Be sure that math assignments completed by our experts will be error-free and done according to your instructions specified in the submitted order form.
Our experts will gladly share their knowledge and help you with programming homework. Keep up with the world’s newest programming trends.

Answer on Other Biology Question for henry

Question #42577
The genome of virus SV40 is a circular doubleā€stranded DNA molecule 5 kb long
(i.e., it contains 10,000 bases)

A 1 kb region is amplified by PCR for four cycles. (Assume that specific
amplification of the target region occurs.) What fraction of the total DNA
does the target sequence constitute?
Expert's answer
During PCR, the region of interestis doubled with each cycle. The end result of four cycles is 24, or 16 copies of the region of interest. 1 kb will produce 16 kb after 4 cycles. The total DNA after 4 cycles will be 16 kb + 4 kb (5kb – 1 kb of target DNA) = 20
kb. The fraction of target sequence will be 16 kb/20 kb=4/5 or 80%.

Need a fast expert's response?

Submit order

and get a quick answer at the best price

for any assignment or question with DETAILED EXPLANATIONS!


No comments. Be first!

Leave a comment

Ask Your question