The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS.
Combine the following:
Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?
Phosphate-buffered saline (PBS) solution is an isotonic buffer (pH 7.4) that is commonly used in experiments involving biological specimens. This is because it mimics the pH, osmolarity, and ion concentrations in the human body, preventing denaturation or degradation of the desired analytes.