Answer to Question #64271 in Molecular Biology for Sanjukta Ghosh

Question #64271
How does RNA polymerase determine which one is the template strand?
Expert's answer
The RNA polymerase itself does not differentiate between two strands of the DNA. Instead, it is guided by the orientation of initiatory complex (composed of transcription factors). Initiatory transcription factors can recognize specific nucleotide sequences on the promoter, therefore, these proteins choose the template strand.

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Assignment Expert
22.12.16, 17:48

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Sanjukta Ghosh
21.12.16, 22:27

In case of prokaryotic RNAP is it sigma factor that specifically binds to -10 and -35 sequences of template strand(promoter)?

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