Below is the protocol to make 1 liter of 10* concentrate PBS.
Combine the following:
14.4g Na2HPO4 (dibasic anhydrous)
2.4g KH2PO4 (monobasic anhydrous)
800ml distilled H2O
adjust pH to 7.4 with HCl
add H2O to 1L
autoclave for 20 minutes on liquid cycle. store at room temperature.
What is the first step to attempt the question, this is my first time doing Biology
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