Answer to Question #262594 in Molecular Biology for Anne

Question #262594

How would you verify that the plasmid contains your insert of interest, in the proper position and orientation?


1
Expert's answer
2021-11-08T17:13:02-0500

Another option for determining insert orientation is to use a primer from within the insert and primers from the plasmid vector for PCR reactions. The two plasmid primers would be located to the left and right of the insert site and pointed toward it.


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