In a laboratory lesson in histology, a student studied a micropreparation at a low magnification of the microscope, and then wanted to examine the structures of interest at a high magnification, but, despite attempts to focus the image, he did not achieve clarity, and the glass of the specimen broke. What mistakes were made when studying the micropreparation?
If you cannot see anything, move the slide slightly while viewing and focusing. If nothing appears, reduce the light and repeat step 4. Once in focus on low power, center the object of interest by moving the slide. Rotate the objective to the medium power and adjust the fine focus only.