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Answer to Question #198595 in Molecular Biology for hen
Question #198595
With reference to the molecular structure of FURA-2, explain why FURA-2 is unable to cross cell membranes?
Expert's answer
Calcium indicators are unable to cross lipid membranes due to their nature, making necessary the use of physical or chemical methods to load them inside the cell. This is the case of Indo-1 AM and Fura-2 AM, which are fluorescent and calcium insensitive.
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