Answer to Question #190940 in Molecular Biology for Munmun

Question #190940

Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.


1
Expert's answer
2021-05-10T17:28:02-0400

Experimental Procedure

  • Do a genomic DNA extraction
  • Run PCR and purify the PCR product: Run PCR to amplify your insert DNA.
  • Digest your DNA:
  • Isolate your insert and vector by gel purification:
  • Ligate your insert into your vector: DNA ligation and transformation of the plasmid DNA into E.coli.
  • Isolate the Finished Plasmid:
  • Verify your Plasmid by Sequencing: DNA sequencing

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Comments

Hasibul
10.05.21, 11:29

Describe how you can clone the gene encoding the protein R in the vector pQE-70. Your description should also indicate the choice of restriction enzymes that can be used, the process of screening of E.coli cells harbouring the recombinant plasmid.

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