We know that DNA polymerase III requires free 3′-OH groups for initiating replication. Would pol-I require free 3′-OH groups when filling in the gaps between Okazaki fragments? If yes, how are free 3′-OH groups provided since primers are removed prior to synthesis
Yes, DNA polymerase I also require free 3' OH group. The free 3'OH end will be provided by the okazaki fragment.