Answer to Question #189898 in Molecular Biology for Misbah

Question #189898

We know that DNA polymerase III requires free 3′-OH groups for initiating replication. Would pol-I require free 3′-OH groups when filling in the gaps between Okazaki fragments? If yes, how are free 3′-OH groups provided since primers are removed prior to synthesis

1
Expert's answer
2021-05-07T14:29:01-0400

Yes, DNA polymerase I also require free 3' OH group. The free 3'OH end will be provided by the okazaki fragment.


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