Answer to Question #148709 in Molecular Biology for Cameron Hicks

Question

Is there a methodology to deciding the correct/optimal substrate/medium in which a tissue culture should be grown. Or do prior tests need to be conducted to find the best medium type?

Answer 1

Yes, there are methodologies that are followed to come up with the best culture media for your research. It is also highly recommended that you do a prior test on several culture media before deciding on the best.

Choosing the best culture medium is the first crucial step in tissue culture technology. To do this, you need first to research on the cell lines you will be using and the different culture media recommended for your cells. Then, you need to decide on whether to use natural or synthetic media.

After that, you need to choose the specific media to use that would offer you quality and consistency at minimal effort. To choose the best culture media the following are taken into consideration:

• The type of cells to be cultured and their specific requirements
• The capabilities and limitations of your Lab
• The purpose of your research

NB: Companies manufacturing various culture media can be consulted because they have valuable information about the specific culture media they have produced. Also ensure you understand your cells, goals, and Lab specifications so that you can make the best choice.