I am trying to understand how to select the best natural/synthetic cell culture media (substrate) so that I can adhere a tissue culture from 2 animals (Catfish and an Electric Eel), is there any good processes to take to decide on the substrate, or can you give me any good recommendations from your knowledge or research papers?? The culture needs to be sustained for a long period of time! Huge thanks
For natural media and for long application time, I recommend Bovine Serum Culture Media. It is the best despite several disadvantages, it has the following advantages;
The disadvantages are;
Despite the many disadvantages, it is still the best natural media.
For synthetic media I recommend a variety such as;
However, it is best to consult the manufactures to give advice based on their own specifications.
Ott, T. (2004). Tissue culture of fish cell lines. NWFHS Laboratory procedures manual, 2, 1-16.