Answer to Question #104875 in Molecular Biology for Kylie

Question #104875
You are working in a molecular biology lab and, unknown to you, your lab partner left
the foreign genomic DNA that you are planning to clone on the lab bench overnight
instead of storing it in the freezer. As a result, it was degraded by nucleases, but still
used in the experiment. The plasmid, on the other hand, is fine. What results would
you expect from your molecular cloning experiment?
1. There will be no colonies on the bacterial plate.
2. There will be blue colonies only.
3. There will be blue and white colonies.
4. The will be white colonies only.
1
Expert's answer
2020-03-13T10:22:15-0400

2. There will be blue colonies only. 

As blue colonies have plasmids without insert whereas white colonies have plasmids with the foreign insert.


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