Answer to Question #9410 in Microbiology for Nauman

Question

Can you please translate this is simple words , i am not a student of biology but have been asked to read a Nature paper , these are lines from it

Parkin is recruited selectively to depolarized mitochondria and directs mitophagy. HeLa cells transfected with HA-Parkin were treated with CCCP for the indicated times( there was a figure in which times were indicated). Mitochondria were stained by anti-TOM20 and a mitochondrial membrane potential-dependent Mitotracker. Parkin was stained with anti-HA. Without treatment mitochondria are intact and stained by both mitochondrial markers.After 2 hours of CCCP treatment, mitochondria are depolarized as shown by the loss of mitochondrial staining. After 24hr of CCCP treatment, massive loss of mitochondria is observed as shown by the disappearance of the mitochondrial marker. Only parkin positive cells show mitochondrial clustering and clearance, in contrast to transfected cells.