Answer to Question #855 in Microbiology for Troy

Question #855
Create an E. coli cell that does not allow for replication of the incA-containing P1 plasmid, but still replicates its own DNA normally. (5 marks) 1. Add a gene to the chromosome that expresses an iteron-binding protein at high levels. 2. Add a gene to the chromosome that expresses a RepA binding protein that prevents RepA from binding to the inc sites. 3. Add a gene that encodes a countertranscript to repA mRNA. 4. Add a gene that binds the host DnaG primase, inactivating it
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