Answer to Question #64888 in Microbiology for Sophie Wilkinson

Question #64888
Results question regarding a serial dilution experiment testing the bacterial coliform content with a 10 fold from 10^-1 to 10^-6. Agar used was CLED, with peptone as a nutrient. If results showed that the most concentrated form (10^-1) showed no bacteria presence after incubation. A good number colonies present (of two types) around a mid-line concentration (10^-3) and then little or no colonies on the least diluted (10^6), how can this be explained? Is this due to the nutrient competition between the bacteria? Thank you in advance
1
Expert's answer
2017-01-30T13:58:14-0500
These results can appear either due to the experimental error or due to the nutrient competition between the bacteria.

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