Answer to Question #38261 in Microbiology for DEVESH KUMAR CHOUKIKAR

Question #38261
Q.20 An enzyme catalyzed reaction (following Michaelis-Menten kinetics) exhibits maximum reaction velocity (Vm) of 75 nmol l -1 min-1 . The enzyme at a substrate concentration of 1.0x10-4 M shows the initial reaction velocity of 60 nmol l -1 min-1 . The Km value of the enzyme in molar concentration (M) is (A) 2.5 x 10-5 (B) 5.0 x 10-5 (C) 2.5 x 10-4 (D) 5.0 x 10-4
1
Expert's answer
2014-01-13T05:14:28-0500
V= Vm*S/(S+Km)
V*S+V*Km=Vm*S
Km=S*(Vm-V)/ V
Km=2.5*10^-5 M
Variant A is correct

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