Answer to Question #31826 in Microbiology for dima

Question

A strain suspension in a turbidity test (spectrophotometer) gave 0.2 . I diluted it to 0.1 equivalent to 10*7 cfu/ml. From this diluted suspension I need to inoculate the two fold dilution tubes with 100 micro liter. However if I add the 10*7 cfu/ml to a 200 micro liter of the antibacterial stock in tube (40 well plates) i may change the strains density, right? if so, shall I take a percentage from the inoculum suspension and how much?? how do i calculate this?