Answer to Question #256135 in Microbiology for Lole

Question

1. List the 4 parameters involved in steam sterilization. (2 marks).
2. What is the difference between “saturated steam” and “super-heated steam”? (3 marks)
3. How is “dry air” removed from the autoclave in the beginning of the cycle? (2 marks)
4. State the functions of the following parts of the autoclave: (6 marks)

• -Safety Valve 1
• Safety Valve 2
• VacuumRelease Valve

5. State 4 precautions that need to be taken when autoclaving “liquid media” in a bottle? (4 marks)

6. What is the purpose of using an ”autoclave tape”? (2 marks)

7. How much water should be added to the autoclave before you start the sterilization process? (1 mark)

Answer 1

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