Answer to Question #193096 in Microbiology for Kim

Question #193096

If the RT-PCR test is negative, give instances when repeat RT-PCR test should be done. If repeated, how many days after the first test should the 2nd test be done?

Expert's answer

Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique combining reverse transcription of RNA into DNA (in this context called complementary DNA or cDNA) and amplification of specific DNA targets using polymerase chain reaction (PCR). it's primarily wont to measure the quantity of a selected RNA. This is often achieved by monitoring the amplification reaction using fluorescence, a way called real-time PCR or quantitative PCR (qPCR). Combined RT-PCR and qPCR are routinely used for analysis of gene expression and quantification of viral RNA in research and clinical settings.The close association between RT-PCR and qPCR has led to metonymic use of the term qPCR to mean RT-PCR. Such use may be confusing, as RT-PCR are often used without qPCR, for example to enable molecular cloning, sequencing or simple detection of RNA. Conversely, qPCR may be used without RT-PCR, for example to quantify the copy number of a specific piece of DNA.

If the RT-PCR test is negative, then repeat RT-PCR test should be done in next 3 days .


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