Can you differentiate Gram positive and Gram negative bacteria using simple staining procedure? Why?
Yes it is possible. The simple staining procedure differentiate between Gram positive and Gram negative bacteria by coloring these cells red or violet. Gram-positive cells have a thick layer of peptidoglycan in the cell wall that retains the primary stain, crystal violet. Gram-negative cells have a thinner peptidoglycan layer that allows the crystal violet to wash out on addition of ethanol. They are stained pink or red by the counterstain.