Answer to Question #157959 in Microbiology for geek

Question

1. Create a “formula” for the process you used above, such that you’d be able to determine the field of view for a lens of any magnification if you knew the field of view of another lens magnification on the same microscope.
2. Using your answers from the previous two questions, estimate the size -- in microns -- of an organism if it covered one-third of the field of view at 100X total magnification. Show your work/process.

Answer 1

1. To calculate field of view, you find the magnification and field number of the microscopes lens in use.

Diameter of microscopes field of view = field number divided by magnification number.

If your microscope uses both an eyepiece and objective lens, multiply the eyepiece magnification by the objective magnification to find total magnification before dividing the field number.

2. Size of an organisms is given by dividing the number of cells in the view with the diameter of the field of view