Answer to Question #137984 in Microbiology for Nosi

Question #137984
Labolatory Identification methods of C Botulinum
Expert's answer
  • Botulism is a rare but serious illness caused by a toxin that attacks the body's nerves and causes difficulty breathing, muscle paralysis, and even death. This toxin is made by Clostridium botulinum and sometimes Clostridium butyricum and Clostridium baratii bacteria.
  • The routine laboratory diagnostics of botulism is based on the detection of botulinum neurotoxin in the patient. Detection of toxin-producing clostridia in the patient and/or the vehicle confirms the diagnosis. The neurotoxin detection is based on the mouse lethality assay.
  • Detecting botulinum toxin can be performed in a liquid such as serum obtained from blood. It can also be detected from the remains of food eaten that has caused a case or an outbreak of botulism. To perform the test using remains of food it is necessary to obtain an extract filtrate from it.

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